mbagchi@life.uiuc.edu
534 Burrill Hall
Office: (217) 244-5054
Lab: (217) 333-7920/3-7697
Fax: (217) 333-1133
Mail to:
Dept of Molecular and Integrative Physiology
524 Burrill Hall
407 S. Goodwin Ave
Urbana, IL 61801
Milan K Bagchi
Professor of Molecular and Integrative Physiology
Education
B.S. 1976 University of Calcutta, India
M.S. 1979 University of Calcutta, India
Ph.D. 1984 University of Nebraska
Postdoc. 1985-89 Baylor College of Medicine, Houston, TX
Teaching Interests
Molecular Pathways of Steroid Hormone Action
The overall goal of research in my laboratory is to gain a clear understanding of the molecular pathways that translate the signals initiated by the binding of steroid hormones estrogen and progesterone to their cognate receptors into biological effects in the target tissue. My laboratory approaches this problem at two levels. First, we aim to understand the mechanisms by which steroid receptors control gene transcription. We are analyzing the function of recently identified chromatin-modifying complexes, coactivators and corepressors, which modulate steroid receptor function in a cell-specific manner. Our recent studies revealed that multiple corepressor complexes interact with estrogen receptor bound to tamoxifen, a well-known hormone antagonist and breast cancer therapeutic. These complexes act in concert to regulate chromatin structure at the estrogen-responsive genes. Functional analysis of these cofactors is essential for deciphering the mechanisms underlying the tissue-specific actions of steroid hormones and will provide important insights that can be utilized for designing better therapeutics for hormone-dependent diseases, such as breast and endometrial cancers. Our second goal is to decipher the gene networks that mediate the physiological actions of estrogen and progesterone in the pregnant uterus, a tissue that is exquisitely responsive to these hormones. Over the past years, we have identified several novel estrogen- and progesterone-regulated genes that control the critical stages of maternal-fetal interactions during early pregnancy. A major effort of the laboratory is directed toward addressing the precise functions of these hormone-regulated pathways, using knockout mouse models and in vitro cell differentiation systems.
Representative Publications
Li Q., Bagchi M.K., Bagchi I.C. (2006) Identification of a signaling pathway involving progesterone receptor, calcitonin and tissue tranglutaminase (tTGase) in Ishikawa endometrial cells. Endocrinology, 147:2147-2154. [Abstract]
Mantena S.R., Kannan A., Cheon Y.P., Li Q., Johnson P.F., Bagchi I.C., Bagchi M.K. (2006) C/EBPbeta is a critical mediator of steroid hormone-regulated cell proliferation and differentiation in the uterine epithelium and stroma. Proc. Natl. Acad. Sci. USA, 103:1870-5. [Abstract]
Bagchi M.K., Mantena S.R., Kannan A., Bagchi I.C. (2006) Control of uterine cell proliferation and differentiation by C/EBPbeta: functional implications for establishment of early pregnancy. Cell Cycle, 5:922-925. [Abstract]
Palanisamy G.S., Cheon Y.P, Kim J., Kannan A., Li Q., Sato M., Mantena S.R., Sitruk-Ware R.L., Bagchi M.K., and Bagchi I.C. (2006) A novel pathway involving progesterone receptor, endothelin-2, and endothelin receptor B controls ovulation in mice. Mol. Endocrinol., 20:2784-2795. [Abstract]
Jeyakumar M., Liu X.F., Erjument-Bromage H., Tempst, and Bagchi M.K. (2007) Phosphorylation of thyroid hormone receptor-associated NCoR corepressor holocomplex by the DNA-dependent protein kinase enhances its histone deacetylase activity. J. Biol. Chem., 282:9312-9322. [Abstract]
Bagchi, I.C., Li, Q., Cheon, Y.P., Mantena, S.R., Kannan, A., and Bagchi, M.K. (2005) "Use of the progesterone receptor antagonist RU 486 to identify novel progesterone receptor-regulated pathways in implantation," Semin. Reprod. Med. 23, 38-45. [Abstract]
Liu, X. and Bagchi, M.K. (2004) "Recruitment of distinct chromatin modifying complexes by tamoxifen-complexed estrogen receptor to natural target gene promoters in vivo," J. Biol. Chem. 279, 15050-15058. [Abstract]
Li, Q., Cheon, Y.P., Kannan, A., Shanker, S., Bagchi, I.C., and Bagchi, M.K. (2004) "A novel pathway involving progesterone receptor, 12/15-lipoxygenease-derived eicosanoids, and peroxisome proliferator-activated receptor gamma regulates implantation in mice," J. Biol. Chem. 279, 11570-11581. [Abstract]
Cheon, Y.P., Demayo, F.J., Bagchi, M.K., and Bagchi, I.C. (2004) "Induction of cytotoxic T-lymphocyte antigen-2 beta, a cysteine protease inhibitor in decidua: a potential regulator of embryo implantation," J. Biol. Chem. 279, 10357-10365. [Abstract]
Cheon, Y.P., Xu, X., Bagchi, M.K., and Bagchi, I.C. (2003) "Immune-responsive gene 1 (Irg1) is a novel target of progesterone receptor and plays a critical role during implantation in the mouse," Endocrinology 144, 5623-5630. [Abstract]
Cheon, Y.P., Li, Q., Demayo, F.J., Bagchi, I.C., and Bagchi, M.K. (2002) "A genomic approach to identify novel progesterone receptor-regulated pathways in the uterus during implantation," Mol. Endocrinol. 16, 2853-2871. [Abstract]